EMPREX M950 MOUSE DRIVER DETAILS:

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EMPREX M950 MOUSE DRIVER



The consequences of repetitive mTBI have become of particular concern for individuals engaged in certain sports or in military operations.

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Many mTBI patients suffer long-lasting neurobehavioral impairments. In order to expedite pre-clinical research and therapy development, there is a need for animal models that reflect the long-term cognitive and pathological features seen in patients.

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In the present study, we developed and characterized a mouse model of repetitive mTBI, induced onto the closed head over the left frontal hemisphere with an electromagnetic stereotaxic impact device. Using GFAP-luciferase bioluminescence reporter mice that provide a readout of astrocyte activation, we observed an increase in bioluminescence relative to the force delivered by the impactor after single impact and cumulative effects of repetitive mTBI. Animals received repetitive mTBI showed a significant Emprex M950 Mouse in spatial learning and memory when tested at 2 and 6 months after injury.

A robust astrogliosis and increased p-Tau immunoreactivity were observed upon post-mortem pathological examinations. These findings are consistent with the deficits and pathology associated with mTBI in humans and support the use of this model to evaluate potential therapeutic approaches. These long-lasting symptoms include memory impairments, difficulty in concentration, depression, apathy, and anxiety 45. Because these symptoms are usually observed in the absence Emprex M950 Mouse significant structural damage, patients sustaining mTBI are difficult to diagnose 6and routine clinical and laboratory evaluations of mTBI patients often fail to show clear morphological brain defects.

Recently, the consequences of repetitive mTBI from multiple concussions have become of particular concern for individuals engaged in certain sports or in military operations because they are at high risk of repeated concussion 78. Military personnel often have several mTBI exposures over the course of their lives and possibly within single deployments 9 Recurrent brain injuries, even when mild, may interfere with neuropsychological recovery 9 Therefore, repetitive mTBI has been associated with greater severity of symptoms, with longer recovery time, and with earlier onset of age-related memory deficits and dementia Repeated concussions have also been associated with chronic traumatic encephalopathy CTEa neurodegenerative disorder with progressive impairments of memory and cognition, as well as depression, anxiety, and motor abnormalities 14 — Our understanding of the mechanisms of these behavioral deficits is still largely incomplete.

Animal Emprex M950 Mouse would certainly facilitate a better understanding of the pathological and behavioral outcomes as a result of concussion 17 — Emprex M950 Mouse, there has been a recent focus in developing animal models of mTBI. A large number of animal models of mTBI have been developed and they have been effective in characterizing the pathological and behavioral changes after acute i.

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Current animal models of concussion have included mild to moderate versions of fluid-percussion impact FPIcontrolled cortical Emprex M950 Mouse CCIand weight drop injury 20 Although these models have demonstrated mild to moderate injury severity levels, most are not capable of mimicking true closed-head Emprex M950 Mouse injury. Furthermore, most of the current mTBI models have been reported to produce some degree of immediate or short-term behavioral deficits; however, it is not clear whether these deficits are long-lasting since few of the published studies made long-term observations beyond 1 month 17 Therefore, our goal of this study was to develop a clinically relevant closed-head injury of repetitive mTBI that results in long-term behavioral and pathological alterations modeling mild brain injury in humans.

To establish injury parameters, we first Emprex M950 Mouse bioluminescence imaging in reporter mice expressing luciferase under the control of a GFAP promoter GFAP-luc mice 22 Bioluminescence imaging of GFAP-luc mice enables us to test a relatively large number of injury conditions in a medium-throughput manner by following astrogliosis and neural injury in the same mice throughout the course of injury. To further validate the long-term cognitive and pathological effects of the injury model, we performed behavioral testing and post-mortem pathological examinations of brain tissue. Two mouse lines were used: Bedding, nesting material, food, and water were provided ad libitum.

Mice were 2—3 months of age at the beginning of experiments. All behavioral testing was performed in isolated behavior rooms. Emprex M950 Mouse

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The Benchmark Stereotaxic Impactor is an electromagnetic stereotaxic impact device capable of producing consistent, graded CCI injuries in Emprex M950 Mouse mice with stereotaxic control of impact location and depth at high velocities Recently it was modified to produce repetitive closed-skull TBI in mice A rubber tip 25 or self-adhesive bumper 3M, St. Mice were anesthetized Emprex M950 Mouse isoflurane 2.

Mice were placed in the stereotaxic frame and secured in prone Emprex M950 Mouse in a customized foam mold, with isoflurane delivered by a gas anesthesia mask Stoelting, Wood Dale, IL, USA Figure 1 A, left panel. The probe tip was fully extended and lowered until the vertex of the bumper touched the scalp Figure 1 A, middle panel. The stereotaxic manipulator was adjusted so that the vertex of the bumper impacted the scalp at a consistent point in relation to the eye and ear of injury side of the head Figure 1 A, right panel.

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The center of impact corresponds to the following coordinates: To induce an impact, the tip was retracted automatically. The Emprex M950 Mouse bar was gently removed to allow the head to move with little or no restraint upon impact.

The stereotaxic device was then moved down, and the electromagnetic device was triggered, driving the tip into the head at a speed and a dwell time set Emprex M950 Mouse the electronics control box. The size and shape of the probe tip and bumper, the distance that stereotaxic device is moved down impact depththe speed the probe tip travels impact speedand Emprex M950 Mouse dwell time can be varied to achieve concussions of different severities. In addition, the location and the angle at which the probe tip impacted the head impact location and angle can be adjusted through the stereotaxic manipulator.

After impact, the mice were allowed to recover from anesthesia on a warming pad and then returned to their home cages.

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Emprex M950 Mouse For sham injuries, the same procedure was performed except that the impact device was discharged in the air; the handling of the Emprex M950 Mouse and duration of anesthesia were identical for both mTBI and sham procedures 24 A mouse model of closed-head mTBI using an electromagnetically controlled stereotaxic impact device and injury severity-dependent activation of a reporter gene. Product Dimensions, 5 x x inches. Item Weight, pounds. Shipping Weight, pounds (View shipping rates and policies). Manufacturer, g: Emprex.

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Product description. Emprex MPU Standard Optical USB Mouse (Black/Metallic Grey). dpi Optical sensor mouse Compact size design for either right or  Missing: M

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